detection of cytolethal distending toxin (cdt) genes in campylobacter jejuni and campylobacter coli isolated from the intestinal of commercial broiler chickens, turkey and quail of iran
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abstract
background: campylobacter jejuni and campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. sharing of the cytolethal distending toxin (cdt) genes in campylobacter is common and is considered species specific. objectives: in this study we focused on detecting the presence of cdt gene in c. jejuni and c. coli isolated from broilers, turkeys and quails of iran. methods: cecal samples were randomly collected from 240 broiler chickens, 100 meat type turkeys and 100 quails after slaughtering. we used pcr as a method for detecting cdt genes. results: in broilers, 93% of 58 c. jejuni positive samples possessed cdt gene and in all cases the three different subunits of cdt genes were present. however, only 56% of 14 c. coli isolates in broilers had contained cdt genes, while one fourth having all three subunits present. in turkeys, around 65% of 34 c. jejuni positive samples had cdt gene present with 38% possessing all three subunits of cdt genes. but all 5 c. coli isolates had all three subunits cdt gene. in quails, 67% of 30 c. jejuni positive samples were identified by cdt gene, 20% of those possessed all three gene subunits. on the other hand, all 28 c. coli isolates of quails had cdt gene present while 36% of those held all three gene subunits. conclusions: our data is indicating the isolation, culture and cdt pcr amplification approaches in this study seemed to be efficient. however, the presence of different variation of campylobacter cdt gene types in our sample isolates signifies the necessity of further functional gene studies to elucidate which gene type combinations result in encoding effective toxins.
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Detection of cytolethal distending toxin (cdt) Genes in Campylobacter jejuni and Campylobacter coli isolated from the intestinal of commercial broiler chickens, turkey and quail of Iran
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Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences betwee...
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Journal title:
iranian journal of veterinary medicinePublisher: دانشگاه تهران
ISSN 2251-8894
volume 9
issue 2 2015
Keywords
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